Journal: The Journal of pathology

Article Title: A 3D tri-culture system reveals that activin receptor-like kinase 5 and connective tissue growth factor drive human glomerulosclerosis

doi: 10.1002/path.4960

Figure Lengend Snippet: (A) MC 3D monoculture without the addition of TGFβ, and (B) in the presence of TGFβ, shown by H&E staining of paraffin-embedded sections. Human collagen type I/III (immunostained with an antibody that recognises both collagen type I and III) (C) and human collagen type IV (D) can be demonstrated in nodules by immunoperoxidase staining. (E) High-power view of MC nodule by scanning electron microscopy in cross section. (F) MC nodule count and collagen type I alpha1 (COL1a1), and collagen type IV alpha1 (COL4a1) RNA quantification (n=3). (G) Effect of ALK5 inhibition (Alk5i) on MC nodule formation (n=3). (H) Effect of SMAD2 or SMAD3 siRNA knockdown in MCs on nodule formation (n=3) with immunoblots demonstrating degree of knockdown. [DharmaFECT (DH), non-targeting siRNA control (siCP), siSMAD2 (siS2), siSMAD3 (siS3), total SMAD2 (tSMAD2), total SMAD3 (tSMAD3)]. Error bars represent 1 s.d. NS, not significant; ***, p<0.001; **, p<0.01: 2-tailed Student’s t test.

Article Snippet: After culture, matrices were embedded in HistoGel, fixed in 4% paraformaldehyde and paraffin wax-embedded for sectioning and staining for human collagen type IV (mouse anti-human collagen IV monoclonal antibody 2150–0121, Bio-Rad, CA, USA) and human collagen type I/III (rabbit anti-human collagen I/III polyclonal antibody 2150–2210, Bio-Rad, CA, USA).

Techniques: Staining, Immunoperoxidase Staining, Electron Microscopy, Inhibition, Western Blot

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Immunohistochemical Analysis of Postburn Scars following Treatment Using Dermal Substitutes

doi: 10.1155/2022/3686863

Figure Lengend Snippet: Histopathological comparison of normal and scar tissue characteristics according to intervention ( n = 63).

Article Snippet: The following antibodies were used: rabbit polyclonal anticollagen III for collagen III (NB100-92162, 200x dilution, enzyme treatment, Novus Biologicals, Littleton, CO, USA), mouse monoclonal antibody for α -SMA (NB600-536, 400x dilution), NB600-408 for collagen I (200x dilution), NBP1-80710 for elastin (200x dilution), NB600-562 for CD31/PECAM (200x dilution, and NBP1-50430 for laminin (Novus Biologicals, Littleton, CO, USA).

Techniques: Comparison

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Immunohistochemical Analysis of Postburn Scars following Treatment Using Dermal Substitutes

doi: 10.1155/2022/3686863

Figure Lengend Snippet: Comparison of elastin fibers. Comparison of elastin fibers of the normal (a), STSG (b), and AlloDerm® (c) groups. 200x magnification, 100 μ m scale bar; immunostaining technique. Elastic fibers are responsible for resilience and recoil in many tissues. There was a significant decrease in elastin fibers in the STSG and AlloDerm® groups compared to the normal group. Therefore, scar tissues in these two groups may be stiffer compared to the other groups.

Article Snippet: The following antibodies were used: rabbit polyclonal anticollagen III for collagen III (NB100-92162, 200x dilution, enzyme treatment, Novus Biologicals, Littleton, CO, USA), mouse monoclonal antibody for α -SMA (NB600-536, 400x dilution), NB600-408 for collagen I (200x dilution), NBP1-80710 for elastin (200x dilution), NB600-562 for CD31/PECAM (200x dilution, and NBP1-50430 for laminin (Novus Biologicals, Littleton, CO, USA).

Techniques: Comparison, Immunostaining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Immunohistochemical Analysis of Postburn Scars following Treatment Using Dermal Substitutes

doi: 10.1155/2022/3686863

Figure Lengend Snippet: Comparison of collagen III fibers. Collagen III fibers of the normal (a) and STSG (b) group; 200x magnification, 100 μ m scale bar; immunostaining technique; Collagen provides tensile strength but hypertrophic scars contain an overabundance of collagen, contributing to a raised appearance and stiffness. There was a significant decrease in collagen III in the STSG group compared to the normal group.

Article Snippet: The following antibodies were used: rabbit polyclonal anticollagen III for collagen III (NB100-92162, 200x dilution, enzyme treatment, Novus Biologicals, Littleton, CO, USA), mouse monoclonal antibody for α -SMA (NB600-536, 400x dilution), NB600-408 for collagen I (200x dilution), NBP1-80710 for elastin (200x dilution), NB600-562 for CD31/PECAM (200x dilution, and NBP1-50430 for laminin (Novus Biologicals, Littleton, CO, USA).

Techniques: Comparison, Immunostaining

Journal: Arthroscopy Techniques

Article Title: All-Arthroscopic Nanofractured Autologous Matrix-Induced Chondrogenesis (A-NAMIC) Technique for the Treatment of Focal Chondral Lesions of the Knee

doi: 10.1016/j.eats.2020.02.007

Figure Lengend Snippet: All-arthroscopic nanofractured autologous matrix-induced chondrogenesis (A-NAMIC) treatment of focal chondral lesions of the knee. Surgery was performed in the left knee joint with the patient positioned supine and using anterolateral and anteromedial portals as visualization and working portals, respectively. (A) Localization of a grade III full-thickness knee chondral lesion (black arrow). (B) Removal of damaged cartilage (black arrow) and sclerotic bone with an open curette. (C) Image showing the spiral configuration of the 9-mm-depth and 1-mm-diameter nanofracture-performed channels (black arrow). The white arrow indicates the well-defined healthy cartilage margins after debridement of damaged cartilage. (D) Implantation of the 3D I/III collagen membrane with 8- and 10-mm-diameter mosaicplasty trephines (black arrow). (E) Final examination to ensure that the lesion is fully covered by the membrane. Black arrows indicate the 2 implants in their final position. (F) Fibrin sealing. The black arrow indicates the final appearance of the lesion after application of the fibrin glue. MC, medial condyle.

Article Snippet: To perform the A-NAMIC surgery, the following instruments and implant were required: the nanofracture device, consisting of a reusable hand instrument, a single-use disposable PleuriStik guide wire, and a single-use thumb tab accessory (Arthrosurface, Franklin, MA); an open 4-mm curette; an arthroscopy bur; 8- and 10-mm mosaicplasty trephines; collagen I/III biphasic matrix (Cartimaix; Matricel, Herzogenrath, Germany); fibrin glue (Tissel, Baxter, Deerfield, IL); ruler; and pen.

Techniques: Membrane

Journal: Arthroscopy Techniques

Article Title: All-Arthroscopic Nanofractured Autologous Matrix-Induced Chondrogenesis (A-NAMIC) Technique for the Treatment of Focal Chondral Lesions of the Knee

doi: 10.1016/j.eats.2020.02.007

Figure Lengend Snippet: Step-by-Step Summary of A-NAMIC Technique

Article Snippet: To perform the A-NAMIC surgery, the following instruments and implant were required: the nanofracture device, consisting of a reusable hand instrument, a single-use disposable PleuriStik guide wire, and a single-use thumb tab accessory (Arthrosurface, Franklin, MA); an open 4-mm curette; an arthroscopy bur; 8- and 10-mm mosaicplasty trephines; collagen I/III biphasic matrix (Cartimaix; Matricel, Herzogenrath, Germany); fibrin glue (Tissel, Baxter, Deerfield, IL); ruler; and pen.

Techniques: Membrane, Saline